五味子提取物的抑菌和杀菌活性研究

Study on the Bacteriostatic and Bactericidal Activity of Extract from Schisandra Chinensis

[目的]探讨五味子提取物的抑菌活性,为其合理开发提供依据。[方法]采用70%乙醇、60%丙酮及沸水3种溶剂对五味子进行搅拌提取,以金黄色葡萄球菌为供试菌,通过平板二倍稀释法测最低抑菌浓度(MIC),试管二倍稀释法计测最低杀菌浓度(MBC)。[结果]五味子3种提取物对金黄色葡萄球菌均有明显的抑制作用,其中60%丙酮提取物的抑制效果最明显,其次是70%乙醇提取物,最后是沸水提取物;MIC分别为25、50和100 mg/ml。同时,低浓度的丙酮提取物抑菌作用不明显,浓度为62.5 mg/ml时细菌的生长被完全抑制,但没有明显的杀菌作用,丙酮提取物的MBC为125 mg/ml。[结论]五味子的丙酮提取物具有明显的抑菌和杀菌作用。

[ Objectives]The aim was to study the bacteriostatic activities of extract from Schisandra chinensis and provide references for its reasonable development. [Method] S. chinensis were stirred and extracted with 3 kinds of solvents such as 70% ethanol, 60% acetone and boiling water. With Staphylococcus aureus as tested bacteria, minimumm inhibition concen. （MIC） was determined by agar doubling dilution method and maximum bacteriostatic concen. （MBC） was measured by tube doubling dilution method. [Result]Three extracts of S. chinensis had significant anti-microbial activity on S. aureus, that of 60% acetone was most obvious, the second was extracts of 70% ethanol and the last was that of boiling water. Their MIC was 25, 50 and 100 mg/ml resp. The bacteriostatic effect of extract of acetone with low concen, was not obvious. The extract at 62.5 mg/ml could inhibit bacterial the growth completely, but had not obvious bactericidal effect. The MBC of extract of acetone was 125 mg/ml. [Conelusion]The acetone extract of S. chinensis had obvious bacteriostatic and bactericidal activity.