不同海拔地区牦牛肌组织线粒体T-AOC的测定

Determination of Mitochondria T-AOC in the Muscle Tissues of Yak in Different Altitude Regions

[目的]为从细胞分子水平进一步探讨牦牛高原低氧适应性的生物学机制及其高原疾病的诊断提供理论依据。[方法]对青海省玛多县和刚察县成年牦牛的心肌、骨骼肌线粒体总抗氧化能力(T-AOC)进行测定。[结果]玛多县牦牛心肌、骨骼肌线粒体的T-AOC分别为(28.94±5.11)和(17.86±5.98)U/mg蛋白,均显著高于刚察县牦牛。在高原低氧环境下,随着海拔高度的增加,牦牛心肌、骨骼肌线粒体T-AOC显著增加。玛多牦牛心肌线粒体的T-AOC显著高于骨骼肌,且差异显著(P<0.05),而刚察牦牛的心肌、骨骼肌线粒体的T-AOC差异不显著(P>0.05)。[结论]牦牛具有对高原低氧环境的显著适应性。

[Objective] Tne research aimed to provide the theoretical basis for further study on the biologieal mechanism of the adaptability of yak to hypoxia in plateau and the diagnosis of its plateau diseases. [Method] Total antioxidant eapaeity （T-AOC） of mitoehondria in cardiac muscle and skeletal muscle of adult yak in Maduo county and Gangcha county of Qinghai province were determined. [Result] T-AOC of mitocbondria in cardiac muscle and skeletal muscle of adult yak in Maduo county were （28.94 ±5.11 ） and （ 17.86 ± 5.98） U/mgprot resp., significantly higher than that in Gangcha county. Under hypobaric hypoxia environment of plateau, T-AOC of mitochondria in cardiac muscle and skeletal muscle of yak were significantly increased with the increasing of altitude height. T-AOC of mitochondria in cardiac muscle of yak in Maduo county was significantly higher than that in skeletal muscle, with significant difference （P 〈0.05）. While T-AOC of mitochondria in cardiac muscle and skeletal muscle of yak in Gangcha county had no significant difference （ P 〉 0.05）. [ Conclusion] Yak had a significant adaptability to hypobaric hypoxia environment of plateau.