孕酮诱发马鹿精子顶体反应的研究

Induction of Sperm Acrosome Reaction Using Progesterone in Wapiti(Cervus elaphus)

以马鹿为试验对象,采用上浮法诱导马鹿精子体外获能,以孕酮(P4)诱导顶体反应,旨在摸索出P4诱发马鹿精子顶体反应的最适宜体系。研究结果表明:0.5～1000μmol/L的P4可提高获能马鹿精子的顶体反应率;其中,1μmol/L与10μmol/L的P4可显著(P<0.05)促进马鹿精子发生顶体反应,并以10μmol/L的P4处理组的顶体反应率最高(74.44±0.71)%。10μmol/L的P4诱导获能马鹿精子顶体反应在10～20min的处理时间内均获得了较高的顶体反应率(均72.41%以上),并以处理10min的顶体反应率最高(78.40%)。P4能促进获能马鹿精子的顶体反应,但不能显著(P>0.05)促进未获能马鹿精子的顶体反应。

Wapiti （Cervus elaphus） sperm was capacitated in vitro through the method of swimming-up and acrosome reaction （AR） was induced by using progesterone （P4） in order to determine the optimal system using P4 during AR. The results showed that： P4 at 0.5 - 1000 μ mol/L could enhance the ratio of AR on the capacitated sperm. Effects of P4 within the range of 1 μ mol/L and 10 μ mol/L were significant （P 〈0.05） on AR and 10 μ mol/L P4 obtained the highest ratio of AR （74.44±0.71）%. Incubated from 10 to 20min by P4 at 10 μ mol/L, the wapiti sperm abtained higher ratio of AR （over 72.41%） and incubated 10min reached the highest ratio （78.40%）. Therefore, AR of capacited wapiti sperm, but not uncapacited sperm （P〉0.05） could be induced by P4.