异丙酚对肠上皮细胞氧化应激损伤的影响

Effects of propofol on intestinal epithelial cells with oxidative stress injury

目的:探讨异丙酚对大鼠肠上皮细胞(intestinal epithelia cell,IEC)氧化应激损伤的影响。方法:利用H2O2产生.OH攻击制备IEC氧化损伤模型。实验设对照组、H2O2组(2.5 mmol/L)、异丙酚组(异丙酚100μmol/L)。用四甲基偶氮唑盐(MTT)实验检测IEC的存活率,测定上清测乳酸脱氢酶(LDH)和细胞中丙二醛(MDA)含量。结果:与对照组相比,H2O2组LDH、MDA水平明显增高,细胞生存率减少(P<0.01)。异丙酚组与H2O2组相比,LDH漏出显著减少,MDA形成减少,并能提高细胞的细胞生存率(P<0.01)。结论:异丙酚对大鼠IEC氧化损伤具有保护作用。

Objective： To study the effects of propofol on IEC in the oxidative stress injury induced by H2O2. Methods： IEC were cultured in intro, which were treated with H2O2. to simulate the IEC injured by OH from H2O2 IEC were divided into three group： control group, H2O2 group （2.5mmol/L） and propofol group （ 100μmol/L）. The IEC viability was observed by 3- （4,5-dimethyhhiazol-2-yl） -2, 5-diphenyhetrazolium bromide （MTT） assay. The lactatc dehydrogenase （ LDH ） that was relased into the culture media and the malondialdehyde （MDA） content in IEC were measured. Results： Compared with the control group, LDH in the culture media and M DA content in H2O2group were significantly elevated, and the IEC viability obviously decreased（ P 〈 0.01 ）. LDH in the culture media and M DA content in propofol group were lower than those of H2O2 group, and the IEC viability was obviously higher than that of H2O2 group. Conclusion： Propofol can protect rat IEC from the oxidative stress injury.