不同因素对油樟愈伤组织生长的影响

Influence of Several Factors on the Growth of Callus of Cinnamomum longepaniculatum(Gamble) N.Chao ex H.W.Li

[目的]筛选油樟组培条件。[方法]以油樟带芽茎段为外植体,通过愈伤组织诱导率、污染率、褐变等指标研究无机盐、光照、抗生素等因素对愈伤组织生长影响。[结果]愈伤组织在2种培养基上均能明显增殖,且MS的培养效果好于1/2MS。由带芽茎段诱导的新生愈伤组织略带绿色。继代培养后,黑暗中诱导和培养的愈伤组织为淡黄色或白色,光照下培养的愈伤组织为褐色。愈伤组织诱导率和外植体污染率都随抗生素浓度的增加而降低,25～50mg/L的链霉素处理出愈率最高(60%～72%),污染率最低(0～15%),死亡率也最低(0)。在相同浓度下,使用链霉素外植体死亡率最低。[结论]MS培养基+25～50mg/L链霉素是油樟组培的最佳条件。

[Objective] The objective of the research was to screen out the condition of tissue culture of Cinnamomum longepaniculatum （Gamble） N.Chao ex H.W.Li. [Method] Taking the C.longepaniculatum stems with bud of as explants, the effect of factors such as mineral salt, illumination, antibiotics on the callus growth were studied according to indexes of callus induction rate, pollution rate and browning etc. [Result] The callus could be obviously proliferated on 2 kinds of mediums. The cultural effect of MS was better than that of 1/2MS. The callus induced from the stems with bud was a little green. After subcuhured, the callus induced and cultured under darkness was amber or white and callus cultured under illumination was brown. The induction rate of callus and the pollution rate of explants were decreased with the increment of antibiotics ~ concentration. When phytomycin was 25-50 mg/L, the forming ratio of callus was highest （60%-72%）, pollution rate （0-15%） and death rate （0） was also lowest. The death rate of explants, treated with phytomycin at the same concentration was lowest. [Conclusion]The optimum condition of tissue culture of C. longepanieulatum was MS medium＋25-50 mg/L phytomycin.