石河子地区棉花黄萎病菌致病型的分子监测

Study on the Molecular Monitoring of the Pathotype of Cotton Verticillium Wilt Pathogen in Shihezi Region

[目的]查明落叶型黄萎病菌在石河子地区的存在情况。[方法]采用了PCR技术对石河子地区棉花黄萎菌系进行检测,监测石河子地区棉花黄萎病菌致病类型。[结果]利用非落叶型棉花黄萎病菌的的特异引物ND1、ND2对石河子棉区采来的114个棉花黄萎病菌菌系进行PCR扩增,108个菌系扩增出1500bp的片段,与文献中报道的非落叶型棉花黄萎菌系扩增出的片段大小一致,说明这些菌系为非落叶型棉花黄萎菌系;而剩余的6个菌系未扩增出任何片段;利用落叶型黄萎病菌的特异性引物D1、D2对这6个菌系进行扩增,也未扩增出任何片段。[结论]非落叶型黄萎病菌占供试菌系的99.5%,表明目前石河子地区棉花黄萎病菌依旧以非落叶型黄萎病菌为主。

[Objective] The aim of the research was to find out the existence of defoliating pathotype of verticillium wilt pathogen in Shihezi region. [Method] Cotton verticillium wilt pathogen in Shihezi region was detected by using PCR technology to monitor the pathotypes of cotton verticillium wilt pathogen in Shihezi region. [Result] The specific primers ND1, ND2 of nondefoliation pathotype of cotton verticillium wilt pathogen were used to make PCR amplification on 114 strains of cotton verticillium wilt pathogen from Shihezi cotton region. The fragment （1 500 bp） was amplified from 108 bacterial system and its size was accordant with the size of the amplified fragment by the nondefoliation pathotype of cotton verticillium wilt pathogen that reported in the literature, which indicated that this bacterial strains were nondefoliation pathotype of cotton verticillium wilt pathogen. While no fragment was amplified form the residual 6 bacterial strains. The specific primers D1 and D2 of defoliating pathotype of verticillium wilt pathogen were used to amplify these 6 bacterial strains and no fragment was amplified also. [Conclusion] The nondefoliation pathotype of cotton verticillium wilt pathogen occupied 99.5% of the tested bacterial strains, which indicated that the cotton verticillium wilt pathogens in Shihezi region was still mainly nondefoliation pathotype of cotton verticillium wilt pathogen at present.