摘要
将苹果杂交种(长富2号×新红星)种子用50mg/LGA3液打破休眠,经消毒处理后接种在MS培养基上,获得无菌组培苗。以该组培苗叶片为试材,通过不同激素组合,获得适宜于植株再生的培养基为MS+NAA0.4mg/L+TDZ2.0mg/L。通过不同质量浓度的Kan筛选,证明15mg/L是Kan最佳的选择。利用含AFL2基因的根癌农杆菌LBA4404转化苹果叶片,得到转AFL2基因的阳性植株,经PCR检测,有2株出现特异性条带,证明该基因已经整合到苹果基因组中。
Dormancy of apple hybrid seeds （Nagafu No. 2 x Starkrimson） was broken by using liquid of 50 mg/L GA3 and sterilized, then cultured in MS medium for getting in-vitro-cultured seedlings. Leaves of tissue cultured seedlings were treated by different hormone concentration. The results showed that the optimum regeneration medium was MS ＋ NAA 0.4 mg/L ＋ TDZ 2.0 mg/L. By screening of Kan concentration, it indicated that the optimum concentration was 15 mg/L. Kanamycin resistance transgenic seedlings were obtained through .4 grobacterium-mediated process which contains A FL2 gene and two of them had specific band by PCR amplification analysis. It was likely to show that A FL2 gene was integrated into the apple genome.
出处
《果树学报》
CAS
CSCD
北大核心
2008年第4期454-457,共4页
Journal of Fruit Science
基金
国家自然科学基金(30671455)
科技部社会公益研究专项(2005DIA4J047)
关键词
苹果
根癌农杆菌
AFL2
转化
Apple seedling
Agrobacterium tumefaciens
A FL2
Transformation
