小麦品种中梁22抗条锈病基因的遗传分析和分子作图

Genetic Analysis and Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Zhongliang 22

对中梁22/铭贤169杂交F2群体苗期抗条锈病鉴定及中国春单体系抗病基因的染色体定位发现,中梁22携带1个显性(暂命名YrZhong22)和1个隐性抗病基因,前者位于5B染色体。由中梁22×铭贤169的F2群体构建抗病、感病池,用SSR标记结合集群分离分析法(BSA),建立了与YrZhong22连锁的4个微卫星标记Xwmc289、Xwmc810、Xgdm116和Xbarc232,并将YrZhong22定位于小麦5BL染色体。YrZhong22与相邻微卫星位点Xwmc810和Xgdm116的遗传距离分别是2.7cM和4.4cM。系谱分析及分子标记分析表明,YrZhong22可能是一个来自中间偃麦草的新抗条锈病基因。

To identify and tag new resistance genes to stripe rust （Puccinia striiform f. sp. tritici） in a wheat （Triticum aestivum L.） cultivar Zhongliang 22 （Zhong 5/S394//Xiannong 4）, resistance identification with the F2 population of Zhongliang 22/Mingxian 169 at seedling stage, monosomic analysis by Chinese Spring, and SSR Markers were employed in the current experiment. Genetic analysis indicated that one dominant nucleic gene （temporarily designated as Yrzhong22） and one recessive cytoplasmic gene conditioned stripe rust rust resistance in Zhongliang 22, and Yrzhong22 was located on chromosome 5B. Using the resistant and susceptible pools derived from a cross of Zhongliang 22 × Mingxian 169, SSR markers combined with bulked segregation analysis （BSA） revealed that four SSR markers located on chromosome 5BL were linked to Yrzhong22. The genetic distances between Yrzhong22 and its nearest makers Xwmc810 and Xgdm116 were 2.7 cM and 4.4 cM, respectively. Yrzhong22 is probably a novel resistance gene to wheat stripe rust donated from Elytrigia intermedium on the basis of pedigree analysis and molecular results.