尼美舒利诱导胃癌细胞凋亡的作用

Effect of nimesulide on inducing apoptosis of gastric cancer cell

目的探讨选择性环氧化酶2(COX-2)抑制剂尼美舒利体外诱导胃癌细胞株SGC7901凋亡的作用和可能的细胞内信号转导机制。方法体外将选择性COX-2抑制剂尼美舒利作用于胃癌细胞株SGC7901,流式细胞仪检测尼美舒利对细胞凋亡的影响;Western blot法检测药物作用前后STAT3蛋白的磷酸化活性(P-STAT3)和bcl-2的表达。结果FCM显示SGC7901细胞经100、200μmol/L尼美舒利作用48h后与对照组相比细胞凋亡百分数增高,且在同一时间随药物浓度增加凋亡百分数增高;Western blot结果显示尼美舒利作用24、48h后P-STAT3、bcl-2蛋白的表达降低。结论尼美舒利体外可促进胃癌细胞凋亡,其机制可能与抑制STAT3信号通路相关蛋白P-STAT3、bcl-2表达有关。

Objective To investigate the effect of apoptosis of gastric cancer cell line SGC7901 treated with selective cyclooxge- nase 2（COX-2） inhibitor nimesulide and the probable mechanism of cell signal transduction. Methods SGC7901 cells were treated with nimesulide. Flow cytometry was used to detect cell cycle and apoptosis. The protein expression related to STAT3 signaling pathway in SGC-7901 cells was examined by Western blot. Results The percentage of apoptosis increased significantly; Western blot analysis showed that protein expression of phosphorylated STAT3 （P STAT3）, bcl-2 in SGC-7901 cells decreased significantly. Conclusion Nimesulide could induce cell apoptosis through down regulating the protein expression of P-STAT3, bcl-2, which is probably one of its molecular mechanisms.