摘要
对福建省流行的PRRS病毒FJ-1的结构蛋白基因进行了克隆、测序.FJ-1结构蛋白基因序列长3188个核苷酸,包含7个开放阅读框(ORF).将FJ-1结构蛋白基因与国内外已发表的18个报道全长结构蛋白基因的PRRSV毒株进行核苷酸序列和推定的氨基酸序列比较,发现:其与17个美洲型毒株核苷酸同源性达到89.7%~92.4%,推定各个ORF编码氨基酸的同源性在85.6%~98.6%之间;而与欧洲型毒株Lelystad核苷酸同源性为54.9%,推定各个ORF编码氨基酸同源性为53.2%~78.2%.遗传进化树分析表明FJ-1与美洲型毒株进化距离近,而与欧洲型毒株进化距离远.从分子水平上证明了福建省流行的PRRS病毒属于美洲型毒株.
Porcine Peproductive and Respiratory Syndrome Virus (PRRSV) FJ - 1 was isolated from a pig farm in Fujian Province of China during an outbreak of PRRS. We determined a 3188 - nucleotide and predicted amino acid sequence of ORFs 2 - 7 gene encoding the structual proteins of FJ - 1, and compared of the structural protein genes of FJ - 1 with the PRRSV strains of 18 fully structural protein genes. It displayed 89. 7 % -92.4 % and 54. 9 % nucleotide identities to 17 North American genotype strains and European genotype Lelystad, respectively, while the deduced amino acids of ORFs 2 - 7 of FJ - 1 showed 85.6 % - 98.6 % and 53.2 % - 78.2 % identities to them, respectively. The phylogenetic tree analysis showed that FJ - 1 was most closely related to the North American genotype strains, but distantly related to the European genotype Lelystad. This study suggests that FJ- 1 belongs to North American genotype.
出处
《集美大学学报(自然科学版)》
CAS
2008年第3期215-220,共6页
Journal of Jimei University:Natural Science
基金
福建省科技攻关重点项目(2003N083)
福建省自然科学基金项目(X0650078)
关键词
猪繁殖和呼吸综合征病毒
福建流行株
结构蛋白基因
序列分析
Porcine Reproductive and Respiratory Syndrome Virus
FJ- 1 isolate
the structural protein genes
sequence analysis