米非司酮合用顺铂对卵巢癌耐药细胞株COC1/DDP增殖及凋亡的影响

Effect of mifepristone combine diamminedichloroplatinum on drug fast ovarian cancer cell lines COC1/DDP proliferation and apoptosis

目的:探讨米非司酮合用顺铂对卵巢癌耐药细胞株COC1/DDP增殖及凋亡率的影响。方法:采用MTT法观察不同浓度的米非司酮合用顺铂对COC1/DDP细胞株增殖活力的影响,采用流式细胞术观察不同浓度的米非司酮合用顺铂对COC1/DDP细胞株凋亡率的影响。结果:对照组和顺铂组比较,米非司酮加顺铂组细胞增殖活力OD值明显下降,细胞凋亡率明显升高,且随着米非司酮浓度的升高变化愈加显著(P<0.05和P<0.01)。结论:米非司酮联合顺铂对COC1/DDP细胞增殖活力均具有显著抑制作用,米非司酮可提高顺铂化疗的敏感性,其增敏作用与促进细胞凋亡有关,且与米非司酮的浓度呈剂量依赖关系。

Objective： To study the effect of mifepristone combine dlamminedichloroplatinum （DDP） on drug fast ovarian cancer eell lines COC1/DDP proliferation and apoptosis. Methods： The effect of various density mlfepristone combine DDP on COC1/DDP cell proliferatlon aetlvlty were observed by MTT method; The effect of various density mifepristone eomblne DDP on apoptosls were observed by FCM method. Results： Compared with control and DDP group, the OD value of cell proliferation aetivlty in mlfepristone eomblne DDP group were decreased obviously, and apoptosis rate were increased, the COC1/DDP eyto- inhibition were changed obviously gradually with increase of rrdfepristone density （ P 〈0. 05 or P 〈0. 01 ） . Conclusion： There are obvious cyto- inhibition on COC1/DDP cell proliferation aetivity by mifepristone eomblne DDP, mifepristone could obviously alleviate the chemotherapy sensitivity of DDP, which were relative to improvement of apoptosis, and the function present the certain relation on the dosage.