摘要
用辐射接枝技术将N-丙烯酰氧基丁二酰亚胺(NASI)接枝在SephadexG75和SephadexG50上。接枝率随剂量增加而增大,10kGy后趋于平缓,剂量率对接枝率无明显影响。两种接枝物分别与牛血清白蛋白(BSA)进行偶合反应。在0.2mol/L、pH=7.4的磷酸盐缓冲液中38℃下恒温缓慢搅拌6h可达偶合平衡。每克BSA可偶合SephadexG75-NASI3.0×10-4mmol。SephadexG50-NASI由于G50型孔隙度过小,BSA无法渗入,偶合反应难以进行。但SephadexG50-NSAI却可与带-NH2、小分子量的中性红发生偶合反应,偶合摩尔比接近1∶1。
N acryloxysuccinimide(NASI) with a function ester group is grafted on Sephadex G75 and Sephadex G50 separately by radiation technology. The radiation grafting conditions including absorbed dose,dose rate,monomer concentration in solvent are investigated. The conjugation reactions between the grafted copolymers Sephadex G75 NASI,and bovine serum albumin(BSA) or Sephadex G50 NASI and bovine serum albumin (BSA)are followed. The experiment results show that the conjugate of Sephadex G75 NASI with larger holes and BSA ( M r=6.6×10 4) is obtained successfully,however,the Sephadex G50 with small holes can be only conjugated with neutral red ( M r=2.58×10 2)。
出处
《同位素》
CAS
1997年第2期65-70,共6页
Journal of Isotopes
关键词
辐射接枝共聚
偶合反应
葡聚糖
NASI
BSA
Sephadex G75 Sephadex G50 N acryloxysuccinimide(NASI) bovine serum albumin(BSA) radiation grafting conjugation reaction