犊牛腹泻大肠杆菌LEE和HPI毒力岛相关基因的检测

Detection of Related Genes of LEE and HPI Pathogenicity Island of Escherichia coli Strains Isolated from Calfs

采用多重PCR方法检测南京部分地区分离的110株犊牛腹泻大肠杆菌中肠细胞脱落位点毒力岛(LEE)和强毒力岛(HPI)。对LEE毒力岛检测其核心区的ler和eaeA基因,对HPI毒力岛检测其irp2和fyuA基因。同时对LEE和/或HPI阳性大肠杆菌进行了O血清型的鉴定。在分离的110株犊牛腹泻大肠杆菌中,具有LEE和/或HPI毒力岛的菌株33株,占30%(33/110)。LEE毒力岛基因检测结果为9.1%(10/110)的菌株ler和eaeA基因的扩增阳性。HPI毒力岛基因检测结果为25.5%(28/110)的菌株irp2和fyuA基因扩增阳性,0.9%(1/110)的菌株irp2阳性。5.5%(6/110)的菌株irp2、fyuA、ler和eaeA基因扩增都为阳性。在33个LEE和/或HPI毒力岛阳性分离株中,O78和O36血清型菌株分别占定型菌株的34.6%(9/26)和27%(7/26)。9.1%的犊牛腹泻大肠杆菌携带LEE,25.5%的菌株携带耶尔森菌HPI,O78和O36为牛源携带LEE和/或HPI毒力岛大肠杆菌常见血清型。

This study was undertaken that adopt the multiplex PCR to determine the present distribution of enterocyte effacement pathogenicity island （LEE） and high pathogenicity island （HPI） among strains of one hundred and ten E. coli isolated from calls in Nanjing. One hundred and ten E. coli isolates were examined for the genes of let and eaeA of LEE, the genes of irp2 and fyuA of HPI by a multiplex PCR, and serogrouped using O antisera of E. coll. Among these E. coli isolates. 30 % （33/110）of the isolates possessed the LEE and HPI. 9.1% （10/110） of the isolates possessed the genes of let and eaeA. 25.5% （28/110） of strains had the genes of irp2 and fyuA. 0.9%（1/110） only had irp2. 5.5% （6/l10）of strains had the genes of irp2, fyuA, let and eaea. In 33 LEE and/or HPI toxicity Island positive isolates, 036 and E. coli 078 serotype strains of stereotypes strains were 34.6 % （9/26） and 27 % （7/26）. 9.1% （10/110） of the isolates possessed the LEE. 25.5 % （28/110） of strains had the HPI. 036 and 078 E. coli is common serotypes that cattle containing LEE and/or HPI pathogenicity island.