曲古抑菌素A诱导胃癌细胞细胞周期阻滞及其机制

TSA induced cell cycle arrest in gastric carcinoma SGC-7901 cells and its mechanisms

目的研究去乙酰化酶抑制剂TSA对胃癌细胞SGC-7901细胞周期的作用及机制。方法利用细胞计数及流式细胞仪检测细胞生长及细胞周期;利用Western blot、基因芯片及实时定量PCR检测TSA对胃癌细胞周期相关基因的表达。结果TSA可诱导胃癌细胞SGC-7901细胞周期阻滞。TSA可增加胃癌细胞SGC-7901之p53,p21,p27等基因的表达,降低CDK2,CCND1等基因的表达。TSA可通过调控多个细胞周期相关基因的表达阻滞胃癌细胞细胞周期,从而抑制胃癌细胞的生长。结论TSA可通过调控多个细胞周期相关基因诱导胃癌细胞细胞周期阻滞,从而达到其抑制胃癌细胞生长的作用。

[Objective] To study the role and mechanisms of cell cycle arrest induced by histone deacetylase inhibitor TSA in gastric carcinoma SGC-7901 cells. [Methods] Cell proliferation assay, PI stain with flow cytometry analysis, Western blot, gene chips, real time PCR were used to study the mechanisms of cell cycle arrest of gastric carcinoma SGC-7901 cell induced by TSA. [Results] ①Histone deacetylase inhibitor （TSA） could induce cell cycle arrest in gastric carcinoma SGC-7901 cell. ②TSA could increase the expressions of p53, p21, p27, etc genes that inhibit cell cycle progress and decrease the expressions of CDK2, CCND1 etc genes that help the cell cycle to progress. ③TSA induced cell cycle arrest through the regulation of multiple cell cycle related genes. [Conclusion] Histone deacetylase inhibitor TSA can induce cell cycle arrest of gastric carcinoma SGC-7901 cells through the regulation of multiple cell cycle related genes.