尖吻蝮蛇毒小分子多肽对人胃癌细胞株SGC-7901的抑制作用

Effect of polypeptide from Agkistrodon acutus venom on adhesion and proliferation of human gastric carcinoma SGC-7901 cell line

目的探讨尖吻蝮蛇毒小肽对体外培养的人胃癌SGC-7901细胞株的增殖抑制作用及作用机制。方法用MTT法观察尖吻蝮蛇毒小肽对人胃癌SGC-7901细胞增殖的影响;采用光镜、电镜观察细胞形态及超微结构变化;荧光染色法测定和观察尖吻蝮蛇毒小肽对SGC-7901细胞凋亡影响;采用结晶紫染色法测定尖吻蝮蛇毒小肽对SGC-7901与FN黏附作用的影响。结果MTT显示尖吻蝮蛇毒小肽呈剂量与时间依赖性抑制人胃癌SGC-7901细胞株的增殖,电镜观察给药组细胞出现明显凋亡,荧光显微镜下可见细胞形态发生改变,尖吻蝮蛇毒小肽对SGC-7901细胞在FN基质上的黏附有一定的抑制作用,并呈浓度依赖关系。结论尖吻蝮蛇毒小肽对体外培养的人胃癌SGC-7901细胞株有较显著的抑制作用,该作用与诱导细胞凋亡和抑制细胞和细胞外基质黏附的能力有关。

[Objective] To investigate the inhibitory effect of a small peptide from Agkistrodon acutus venom on the growth of human gastric carcinoma SGC-7901 cell line and its mechanism. [Methods] The SGC-7901 cells were treated with the small peptide at different concentrations. The proliferation of SGC-7901 cells was assayed with MTr colorimetric method. The histological and ultrastructural changes of the cells were scored using light and electron microscopy.The apoptosis of SGC-7901 cells induced by the small peptide was studied by fluorescent staining, Crystal violet staining and MTr assay were employed to determine the effect of the small peptide on adhesion of SGC-7901 cells to fibronectin. [Results] Proliferation of SGC-7901 cells was inhibited significantly by a small peptide from Agkistrodon acutus venom in a dosage and time dependant manner. Under electron-microscope and fluorescent microscope some SGC-7901 cells underwent typical apoptosis and morphological changes after 24 hours＇ incubation with the small peptide. The small peptide could inhibit the adhesion of SGC-7901 cells to fibronectin in a dose-dependent manner. [Conclusion] A small peptide can inhibit proliferation of SGC-7901 cells ,which may be related to the mechanism of inducing cellular apoptosis and inhibiting cellular adhesion.