摘要
原生质体融合技术是细菌遗传育种的有效方法之一,发展迅速,应用广泛。文中综述了亲本菌株选择性遗传标记方法、影响原生质体制备与再生因素、原生质体融合方法和条件。介绍了细菌原生质体融合技术在遗传育种中的应用,并展望了细菌原生质体融合技术的发展前景。
The protoplast fusion technique was one of the most effective ways of bacteria genetic breeding, meanwhile it developed rapidly and was widely used. This paper summarized the method of selective genetic marks, the factors that affected the formation and regeneration ofprotoplasts and the ways and conditions of protoplast fusion. In addition, the application of bacteria protoplast fusion technique in genetic breeding was reviewed in the article and the development of this technique was looked forward to.
出处
《中国酿造》
CAS
北大核心
2008年第4期1-6,共6页
China Brewing
基金
黑龙江省普通高等学校骨干教师创新能力资助计划(1055G063)
齐齐哈尔市农业攻关及推广计划项目(NG06-44)
关键词
原生质体融合
原生质体制备
原生质体再生
遗传育种
protoplast fusion
protoplast preparation
protoplast regeneration
genetic breeding
