摘要
采用生物化学方法测定和分析了河川沙塘鳢(Odontobutis potamophila)胚胎、仔鱼发育过程中脂类的含量及脂肪酸的组成。结果显示,随着胚胎和仔鱼的发育,其总脂的含量呈下降趋势,饱和脂肪酸(SFA)的含量亦呈现出下降趋势,单不饱和脂肪酸(MUFA)的含量在胚胎期保持稳定、在仔鱼期下降,而多不饱和脂肪酸(PUFA)却呈现出不断增长的趋势。在整个胚胎和仔鱼发育过程中,平均含量最高的脂肪酸依次是C16∶0、C18∶1n-9、C18∶0、C16∶1、C22∶6n-3(DHA)和C20∶5n-3(EPA)。结果表明:河川沙塘鳢在胚胎和仔鱼发育过程中有消耗饱和脂肪酸和单不饱和脂肪酸,而保存n-3系列和n-6系列的高度不饱和脂肪酸的趋势,饱和脂肪酸被作为胚胎期能量代谢的主要来源,单不饱和脂肪酸被作为仔鱼期能量代谢的主要来源,而C18∶3n-3(亚麻酸)和C18∶2n-6(亚油酸)被用于合成DHA、EPA和C20∶4n-6(AA)。
The content of lipid and the composition of fatty acid were measured and analyzed during the embryonic and larval development of Odontobutis potamophila. The results indicated that the content of total lipid decreased during the embryonic and larval development. Saturated fatty acids（SFA）also decreased with development. Monounsaturated fatty acids （MUFA） remained stable during embryonic development, which decreased significantly during larval development. Polyunsaturated fatty acids（PUFA） increased significantly with development. The fatty acids that occurred in the highest abundance in all developmental stages, in order, were C16： 0, C18： 1 n-9, Cl8： 0, C16：1 , C22：6n-3 （DHA）and C20：5n-3 （EPA）. Overall, there was a tendency to consume saturated fatty acids（SFA） and monounsaturated fatty acids（MUFA） , and conserve n-3 and n-6 series highly unsaturated fatty acids（HUFA）. Saturated fatty acids（SFA） were utilized as energy substrates during embryonic development, and monounsaturated fatty acids（MUFA） were utilized as energy substrates during larval development, C18：3n-3 （linolenic acid）and C18： 2n-6（ linoleic acid）were utilized to synthesize DHA, EPA and C20： 4n-6（AA）.
出处
《淡水渔业》
CSCD
北大核心
2008年第3期46-50,共5页
Freshwater Fisheries
基金
上海市科学技术委员会重大项目(04DZ19301)
重庆师范大学重点科研项目(08XLB001)
