太平洋蓝鳍金枪鱼野生群体遗传多样性的初步研究

Primary Research of Genetic Diversity of the Natural Stock of Pacific Bluefin Tuna (Thunnus thynnus)

采用RAPD和微卫星（simple sequence repeats，SSRs）分子标记技术对太平洋蓝鳍金枪鱼（Thunnus thynnus）野生群体遗传多样性进行了分析．运用18个随机引物进行RAPD分析，共扩增产生96个可统计条带，其中多态性条带32条，多态位点百分率为33．3，Shannon’s遗传多样性指数0．2430，群体平均杂合度H0．1926；在SSRs分析中筛选出10个SSRs引物，多态座位比例为100％，Shannon’s遗传多样性指数1．1622，群体平均杂合度0．6486．多态微卫星位点的多态信息含量（PIC）为0．3571～0．8187，平均0．5462，分析结果表明太平洋蓝鳍金枪鱼的群体遗传多样性处于较高水平．

The genetic diversity of wild Pacific bluefin tuna（Thunnus thynnus） Was investigated by using the simple sequence repeats （SSRs） and randomly-amplified polymorphic DNA （RAPD）. Of the random primers screened, 18 produced highly reproducible RAPD bands. A total of 96 discernible DNA fragments were detected,of which 32 being polymorphic. The percentage of polymorphic, Shannon＇s index and the average heterozygosity were 33.3 % ,0. 243 0 and 0. 192 6 respectively; Of the SSRs primers screened, 10 produced highly reproducible SSRs bands. The percentage of polymorphic,Shannon＇s index and the average heterozygosity were 100%, 1. 162 2 and 0. 648 6 respectively. The polymorphic information content （PIC） of polymorphic loci varied between 0. 357 1- 0. 818 7, and the average PIC was 0. 546 2. It seemed that genetic diversity of the Pacific bulefin tuna population was at a high level.