铜绿假单胞菌PA1550基因对泳动及蹭动的影响

Impact of Pseudomonas aeruginosa gene PA1550 on its swimming and twitching motility

【目的】:研究与铜绿假单胞菌运动能力相关的基因。【方法】:以一株临床分离的铜绿假单胞菌PA68做受体菌,应用人工Mu转座技术建立了库容为2000的突变子文库,从中筛选出泳动能力和蹭动能力丧失或减弱的突变子,通过基因克隆、测序,GenBank BLAST比对测序结果,互补基因表达确定与铜绿假单胞菌运动能力相关的基因。【结果】:突变子Y46在丧失了泳动运动能力的同时,蹭动能力也发生了减弱。在Y46突变子中,Mu转座子插入到功能完全未知的基因PA1550中。对极性效应及PA1550所在操纵子的分析表明,Mu转座子对插入点下游的基因的转录并不造成影响。【结论】:PA1550与铜绿假单胞菌的泳动及蹭动能力有关。

[Objective] Studying the genes involved in swimming and twitching motility of Pseudomonas aeruginosa. [Methods] We used Mu transposition technique, gene cloning, nucleotide sequencing and the trans-complementation experiment to study the genes involved in twitching motility and swimming motility of P aeruginosa strain PA68 isolated from a patient with bronchiectasis. [Results] A mutant deficient in both swimming and twitching motility was isolated out of about 2000 mini-Mu insertion mutants. The result of GenBank BLAST showed that the mini-Mu transposon had inserted into the gene PA1550 with unknown function. Analyses on the operon of PAl550 and the polar effect revealed that Mu transposon had no effect on the transcription of the downstream genes of PA1550. [Conclusion] PA1550 is involved in the swimming and twitching motility of Pseudomonas aeruginosa.