苏云金芽胞杆菌产苏云金素菌株CT-43及其缺失突变株的差异蛋白

Differential expression protein between the thuringiensin-yield Bacillus thuringiensis strain CT-43 and its mutants

【目的】苏云金素(Thuringiensin)的合成和代谢途径的相关研究在国内外一直进展缓慢,本文拟从蛋白质组水平揭示与苏云金素合成或代谢相关的蛋白。【方法】利用双向电泳技术研究了高产苏云金素的苏云金芽胞杆菌野生菌株CT-43、其高产突变菌株CT-43-1C及不产突变菌株BMB0806在蛋白表达水平的差异,然后对差异蛋白点进行质谱鉴定,最后对鉴定出的蛋白进行生物信息学分析。【结果】与野生型和高产菌株相比,在BMB0806中发现了13个差异显著的蛋白点,鉴定出了其中的9个,生物信息学结果显示有6个蛋白可能与苏云金素合成或代谢相关。【结论】通过蛋白质组研究找到了6个可能与苏云金素合成或代谢相关的蛋白,为苏云金素合成基因簇的克隆和合成途径的验证提供了有力的证据。

[Objective] The purpose of this research is trying to uncover the biosynthetic or metabolic relative protein of thuringiensis by proteomics. [Methods] We conducted differential expression analysis between the high thuringiensin-yield Bacillus thuringiensis strain CT-43 and its mutants, high production strain CT-43-1C and non-production strain BMB0806, based on the 2-D gel. Then through mass spectrum （MS） identification and bio-informatics we analyzed the detected proteins. [Results] Thirteen spots were selected to be detected by the MS, and nine of them were identified. Among them, six proteins including in the basal metabolism pathway were possibly deduced that relative with the synthesis or metabolism of thuringiensin production. [Conclusion] There were six proteins found to be connected with the influential role protein of biosynthesis and metabolic of thuringiensin production by comparative proteomics. This research provides the evidence of thuringiensis gene cluster cloning and biosynthetic analysis.