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利用鼠尾胶原建立胶原微滴药物敏感性检测方法 被引量：2

Establishing the method of collagen-gel droplet embedded culture drug sensitivity test with rat tail collagen
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摘要 目的利用鼠尾胶原建立胶原凝胶微滴肿瘤药物敏感性检测（CD—DST）技术，探讨其在胰腺瘤等恶性肿瘤化疗敏感性检测中的应用。方法利用鼠尾胶原建立CD—DST检测方法，对3株胰腺癌细胞株、15例胰腺癌和10例胃肠道恶性肿瘤的手术切除标本进行临床化疗药物敏感性检测。结果利用鼠尾胶原建立的CD—DST能有效的对胰腺癌细胞株和临床肿瘤标本进行体外肿瘤药物敏感性检测，对临床肿瘤标本的整体检测成功率为80％（20／25），胰腺癌细胞在体外对5-氟尿嘧啶、吉西他滨、奥沙利铂的化疗敏感性低于胃肠道恶性肿瘤。结论CD—DST是一种切实可行的体外肿瘤化疗药物敏感性检测方法，可用于临床制定个体化化疗方案。 Objective To explore the clinical application of collagen-gel droplet embedded-culture drug sensitivity test （CD-DST） in the malignant tumors. Methods CD-DST was established with rat tail collagen. Three pancreatic cancer cell lines, surgical resection specimens including 15 cases of pancreatic cancer and 10 cases of gastrointestinal cancer were examined using CD-DST. Results The overall achievement ratio of CD-DST for clinical tumor specimens was 80% （ 20/25 ）. In vitro chemosensitivities of pancreatic carcinoma cells to 5-FU, gemcitabine and oxaliplatin were lower than those of gastrointestinal carcinoma. Condtmions CD-DST with rat tail collagen is a valuable chemosensitivity testing method for malignant tumors. It can be used to realize individualized anticancer chemotherapy.
作者 胡亚 周涛 廖泉 张太平 陈革 赵玉沛
机构地区 中国医学科学院中国协和医科大学北京协和医院基本外科
出处 《中华外科杂志》 CAS CSCD 北大核心 2008年第13期998-1001,共4页 Chinese Journal of Surgery
基金 国家自然科学基金资助项目（30772526）
关键词 抗药性 肿瘤 肿瘤 实验性 胶原微滴肿瘤药敏检测 Drug resistance, neoplasms Neoplasms, experimental Collagen-gel droplet embedded-culture drug sensitivity test
分类号 R686 [医药卫生—骨科学]
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参考文献13

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同被引文献22

  • 1徐鹏,章克昌,王玉红,夏咏梅.紫外光谱法评价27种中药的防晒效果[J].精细化工,2006,23(4):375-377. 被引量：26
  • 2梁智,田海梅,李艳芬,曹冬艳,刘毅,李茉,王小兵,程冬婉,张伟.胶滴肿瘤药敏检测技术(CD-DST)的建立及初步临床应用研究[J].癌症进展,2006,4(6):545-549. 被引量：11
  • 3朱慧敏,陈志.天然产物抗紫外线的研究进展[J].青海科技,2007,14(1):49-52. 被引量：7
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中华外科杂志
2008年 第13期

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