依那普利拉减轻大鼠烧伤早期脏器损害的研究

Protective effect of enalaprilat injection against early postburn organ damage in rats

目的探讨依那普利拉对烧伤早期内脏损害保护作用的量效关系。方法随机将54只SD大鼠分为烫伤组（B组）和烫伤后小剂量、中剂量和大剂量依那普利拉治疗组（E1、E2、E3组），每组12只大鼠，另取6只大鼠不致伤作为正常对照组。检测烫伤前及伤后6h和12h动脉血压、血管紧张素Ⅱ、尿素氮（Bun）、肌酐（Cr）和肌酸激酶（CK）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST），以及主要脏器组织病理变化。结果B组血管紧张素Ⅱ明显升高，治疗组均明显低于B组（P均〈0．05），E2和E3组明显低于E1组；B组大鼠CK，Bun、Cr，ALT，AST均显著高于正常参考值（P均〈0．05），治疗组较B组不同程度降低，E1组下降明显（P〈0．05），各时相点基本与正常值接近；E2和E3组下降不明显，明显高于E1组；烫伤后各组动脉收缩压和舒张压均有不同程度下降，其中E2、E3组较E1组更明显，E1组较B组明显升高。结论小剂量依那普利拉能够显著减轻严重烧伤后脏器损害的程度，但对动脉收缩压和舒张压无显著影响。

Objective To investigate the dose-effect relationship of enalaprilat（ENA） injection on the organ damage following early burn injury in rats. Methods A total of 54 SD rats were subjected to 30% total body surface area Ⅲ scald injury, and were randomly divided into simple scald group （B group, with conventional fluid transfusion after scald）, ENA treated group （E1, E2, E3 group, with intraperitoneal enalaprilat injection of 1,2, 4 mg/kg after scald respectively ）. Other 6 rats were taken as normal control. Aortic systolic pressure （AOSP）, aortic diastolic blood pressure （AODP）, mean arterial pressure （MAP） , angiotensin Ⅱ , blood urea nitrogen （ Bun ） , creatinine （ Cr ） , creatinine kinase （ CK ）, alanine aminotransferase（ALT） ,asparate aminotransferase（AST） of the simple scald group, E1 group,E2 group and E3 group were investigated at 6 h and 12 h post burn. Results Ang Ⅱ , Bun, Cr, CK, ALT, AST levels in ENA treated group after 6 h and 12 hours were significantly lower than those of simple scald group （ all P 〈 0. 05 ）. AOSP, AODP, MAP in ENA treated group after 6 and 12 hours were significantly higher than those of simple scald group （ all P 〈 0. 05）. Conclusion Low-dose enalaprilat, injection （ 1 mg/kg） could alleviate organ damage in post-burned rats, but has little effect on AOSP and AODP.