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天然羟基磷灰石/壳聚糖复合材料细胞相容性研究 被引量：2

Cellular biocompatibility of nature hydroxyaptite/chitosan composite
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摘要 目的:评价天然羟基磷灰石/壳聚糖复合材料(nature hydroxyaptite/chitosan composite,NHC)的细胞相容性。方法:人骨髓间充质干细胞诱导法培养成骨细胞,并鉴定。将材料与细胞体外进行复合培养,应用细胞增殖度,碱性磷酸酶和扫描电镜等对其细胞相容性进行评定。结果:骨髓间充质干细胞诱导的成骨细胞在复合材料表面粘附,生长良好,细胞增殖度无显著性差异(P>0.05),材料组与对照组之间碱性磷酸酶无显著性差异(P>0.105),扫描电镜观察发现24h后细胞生长良好,细胞连接紧密,有细胞外基质生成。说明本复合材料具有良好的细胞相容性。结论:NHC具有较良好的体外细胞相容性。 Objective To investigate the cellular biocompatibility of nature Hydroxyaptite/ Chitosan composite （NHC）. Methods The composite was cocultured with osteoblasts derived from human bone marrow stem cells. The cellular biocompatibility was studied by means of the evaluation of proliferation capacity, alkaline phosphatase staining and scanning electron microscope observation. Results Excellent adherence and growth of osteoblasts were observed on the composite surface. There were no significant differences in both cell proliferation capacity and alkaline phosphatase staining between the experiment and control groups, SEM observation showed normal growth of osteoblasts and extracellular matrix generation in 24 hours. Conclusion NHC had good biocompatibility with the osteoblasts in vitro.
作者 唐晓军 归来 吕晓迎
机构地区 北京协和医学院中国医学科学院整形外科医院颅颌面外科 东南大学生物科学与医学工程学院生物电子学国家重点实验室
出处 《中国美容医学》 CAS 2008年第6期855-858,共4页 Chinese Journal of Aesthetic Medicine
基金 生物电子学国家重点实验室开放课题资助
关键词 羟基磷灰石 壳聚糖 细胞相容性 人骨髓间充质干细胞 hydroxyaptite chitosan cellular biocompatibility human bone marrow stem cells
分类号 Q813.1 [生物学—生物工程]
  • 相关文献

参考文献9

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共引文献6

同被引文献31

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引证文献2

二级引证文献5

中国美容医学
2008年 第6期

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