纤维蛋白胶促进急性完全横断性脊髓损伤修复的初步研究

THE EFFECTS OF FIBRIN GLUE ON ACUTE COMPLETE TRANSECTION SPINAL CORD INJURY

目的探讨纤维蛋白胶(fibrin glue,FG)对完全横断性脊髓损伤修复和再生的影响。方法取健康雌性SD大鼠10只,体重250～300g,制备急性完全横断性脊髓损伤模型。随机将其分为实验组和对照组,每组5只,实验组脊髓断端注入FG(100μL/只),对照组不予任何治疗。术后4周,采用BBB运动评分法进行运动功能评价。术后4周处死动物,应用免疫组织化学方法观察神经中丝(neurofi lament,NF)和神经胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)表达。采用图像分析方法,对脊髓横断处远、近端进行神经纤维计数及GFAP面积比分析。结果BBB运动评分:对照组(2.40±0.51)分,实验组(3.00±0.45)分,两组间运动功能比较差异无统计学意义(P>0.05)。免疫组织化学观察示,对照组近、远端可见少量NF阳性细胞和GFAP阳性结构;实验组近、远端可见大量NF阳性细胞及GFAP阳性结构生长,并向损伤中心区汇聚,但未到达中心区。图像分析显示,神经纤维数量:实验组近端(113.10±20.75)根、远端(73.60±33.61)根,对照组近端(45.50±17.18)根、远端(23.50±8.20)根;GFAP面积比:实验组近端33.75%±11.06%、远端27.75%±7.15%,对照组近端23.78%±5.76%、远端19.78%±5.17%;实验组与对照组比较差异均有统计学意义(P<0.05)。结论FG对脊髓损伤具有一定程度的修复及促进其再生的作用。

Objective To investigate the effects of fibrin glue on repair and regeneration of acute complete spinal cord injury. Methods Acute complete transaction spinal cord injury model were made in 10 adult healthy SD rats（female, weighing 250-300 g）, randomized grouping： treated group（n=5） and control group（n=5）. In the treated group, fibrin glue was implanted covering on the injury site and filling the lesion gap. In the control group, no treatment was given. At 4 weeks, the locomotor functions of the rats were detected by basso, beattie and bresnahan （BBB） score, then the means of immunohistochemistry were used to observe neurofilament（NF） and glial fibriUary acidic protein（GFAP）. And image analysis was used to measure the quantify of the nerve fiber and the fibers area ratio of astrocyte. Results The BBB scores were 2.40 ±0.51 in control group, 3.00± 0.45 in treated group, showing no significant difference （P 〉 0.05）. By immunohistochemistry： a little positive NF cells and GFAP frame were found in control group; more positive NF cells and GFAP frame were found in treated group, the cells and frame grew toward the center but did not arrive at the center. Image analysis showed the amount of never fibers in treated group （rostral region： 113.10 ± 20.75, caudal region： 73.60 ± 33.61） was more than that in control group （rostral region： 45.50 ± 17.18, caudal region 23.50 ± 8.20）, showing significant difference. The fibers area ratio of astrocyte in treated group（rostral region： 33.75% ± 11.06%, caudal region： 27.75% ± 7.15%） was more than that in control group（rostral region： 23.78%±5.76%, caudal region： 19.78% ± 5.17%）, showing significant difference （P 〈 0.05）. Conclusion Fibrin glue can promote repair and regeneration of acute spinal cord injury.