庚型肝炎病毒 C 型(GBV-C)多肽的抗原性研究及应用

Evaluation of Peptide Antigens for Detection of Antibodies to GBV C

应用Ｇｏｌｄｋｅｙ软件分析ＧＢＶ－Ｃ的氨基酸序列，采用标准的Ｍｅｒｉｆｉｅｌｄ固相法和多中心同步多肽合成技术研究ＧＢＶ－Ｃ多肽抗原，应用抗原反应性好的ＮＳ３区Ｐ１和Ｐ１１多肽以及ＮＳ５区的Ｐ２２多肽研制抗ＧＢＶ－Ｃ检测试剂。９９例ＧＢＶ－ＣＲＮＡ阳性血清的抗ＧＢＶ－Ｃ阳性率为８１．８２％（８１／９９），１０００例单采浆献血员血样的抗ＧＢＶ－Ｃ阳性率为５．１０％（５１／１０００），抗ＧＢＶ－Ｃ阳性血样中的ＧＢＶ－ＣＲＮＡ阳性率为７０．５９％（３６／５１）。结果表明，Ｐ１，Ｐ１１和Ｐ２２可以用于抗ＧＢＶ－Ｃ检测试剂的开发。应尽快对献血员，尤其是献浆员进行抗ＧＢＶ－Ｃ的筛选。

Peptide antigens were synthesized and analyzed for detection of antibodies to GBV C.Antigenicity was found in 60 71% synthesized peptides (17/28).P1,P11(derived from NS3 region)and P22(derived from NS5 region) were used as the solid phase antigens for EIA.Anti GBV C were found in 81 82%(81/99) GBV C RNA positive samples and in 5 10%(51/1000) plasma donors.The results showed that P1,P11 and P22 were useful for detection of antibodies to GBV C.GBV CRNA was detected in 70 59% anti GBV C positive plasma donors,suggesting that GBV C is an agent of post transfusion hepatitis and screening blood donors with anti GBV C diagnostic reagents is important for control of GBV C infection.