T 细胞受体 Vβ基因在识别 HSV-2 过程中的表达水平和特点

Expression of T cell receptor Vβ genes of PBLs stimulated with HSV 2 in vitro

本课题主要研究Ｔ细胞受体（ＴＣＲ）识别抗原后Ｖβ基因发生重排，ｍＲＮＡ表达水平改变。用紫外线处理的单纯疱疹病毒－２型（ＨＳＶ－２）、ＨＳＶ－２及ＰＨＡ分别感染或刺激正常人的ＰＢＬｓ，培养４～６天后，提取ｍＲＮＡ，并采用ＲＴ－ＰＣＲ、Ｓｏｕｔｈｅｒｎ杂交发现识别ＨＳＶ－２的ＴＣＲＶβ２、６、７、８基因在体外选择性扩增。在采用ＨＳＶ－２攻击皮肤病患者发作期、缓解期以及再发作期的ＰＢＬｓ时，发现ＴＣＲＶβ基因表达随着病程变化而改变，尤其Ｖβ７亚家族表达水平显著高于其它亚家族基因，这显示了ＴＣＲＶβ基因的变化是恒定特异性的。

Human peripheral blood lymphocytes(PBLs) of healthy people and patients were infected or stimulated with herpes simplex virus(HSV 2),ultraviolet radiated HSV 2(HSV 2 UV) and PHA,respectively and cultured separately for 4 to 6 days.The mRNAs from the PBLs were extracted separately and the expression level of T cell receptor (TCR) Vβ gene were determined.The results showed that TCR Vβ6,7 and 8 genes were selectively amplified.The expression levels of different genes of TCR Vβ varied at different clinic stages when stimulated with HSV.It suggests that the expression of TCR Vβ genes is of absolute specificity.