(+)-松萝酸治疗小鼠急性弓形虫病的初步研究

A Preliminary Study on the Effect of (+)- Usnic Acid on the Treatment of Acute Toxoplasmosis in Mice

目的探索治疗弓形虫病的有效药物;初步观察(+)-松萝酸治疗小鼠急性弓形虫病的疗效。方法小鼠腹腔接种RH株弓形虫速殖子500个,建立急性弓形虫病动物模型;设立(+)-松萝酸治疗组(50 mg/kg,每日2次)、乙酰螺旋霉素治疗组(50 mg/kg,每日2次)、DMSO及淀粉对照组(含1%DMSO的5%淀粉溶液0.5ml,每日2次),分别于感染2 h后灌胃,每日2次,连续10 d,观察对比疗效。结果50 mg/kg(+)-松萝酸治疗组明显延长了小鼠的存活时间,最长存活时间达15 d,平均存活时间为10.63 d;而螺旋霉素治疗组小鼠最长存活时间为10 d,平均为7.37 d;对照组仅5.42 d。(+)-松萝酸治疗组与螺旋霉素治疗组及对照组之间均有显著性差异。结论(+)-松萝酸具有治疗急性弓形虫病的潜在作用,并且疗效优于螺旋霉素。

Objective To evaluate the efficacy of （＋）-usnic acid in the treatment of acute Toxoplasmosis in mice. Method Mice were infected intraperitonellay with 500 tachyzoites RH strain of Toxoplasma gondii. Two hours after infection, the mice were divided into （＋）-usnic acid treatment group （50 mg/kg, twice per day）, acetylespiramycin treatment group （50 mg/kg, twice per day） and control group （1% DMSO and 5% amylum solution）. The mice were administered with drug or DMSO / amylum solution by garage for 10 consecutive days. Result The survival rate of mice in the （＋）-usnic acid treatment group was increased and the average survival time in mice of the （＋）-usnic acid group, acetylespiramycin treatment group, and control group was 10.63, 7.37, and 5.42 days, respectively. Significant difference in the curative effect was observed among （＋）-usnic acid treatment group, acetylespiramycin treatment group, and the control group. Conclusion （＋）-usnic acid is effective in the treatment of acute Toxoplasmosis.