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Effect of Quercetin on Proliferation and Apoptosis of Human Nasopharyngeal Carcinoma HEN1 Cells 被引量：5

Effect of Quercetin on Proliferation and Apoptosis of Human Nasopharyngeal Carcinoma HEN1 Cells
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摘要 The effect of quercetin （Que） on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells was investigated. Inhibition rate of quercetin on HEN1 was assayed by MTT method, apoptosis by flow cytometry （FCM）, and the caspase-3 expression of each group by colorimetry set respectively. Quercetin inhibited HEN1 cells in in a dose-（r=0.709, P〈0.01） and time-dependent manner （r=0.703, P〈0.01）. The ratio of apoptotic and necrosis cells was increased in the cells treated with quercetin. Cell cycle was specificly arrested in G2/M phase. Apoptosis cusp was revealed by FCM. The activity of caspase-3 was significantly up-regulated in 5 groups treated with quecetin as compared with control group （P〈0.05）. It was concluded that the growth inhibition of quercetin was highly related to cell cycle arrest at the G2/M phase and induction of caspase-dependent apoptosis in human nasopharyngeal carcinoma HEN 1 cells. The effect of quercetin （Que） on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells was investigated. Inhibition rate of quercetin on HEN1 was assayed by MTT method, apoptosis by flow cytometry （FCM）, and the caspase-3 expression of each group by colorimetry set respectively. Quercetin inhibited HEN1 cells in in a dose-（r=0.709, P〈0.01） and time-dependent manner （r=0.703, P〈0.01）. The ratio of apoptotic and necrosis cells was increased in the cells treated with quercetin. Cell cycle was specificly arrested in G2/M phase. Apoptosis cusp was revealed by FCM. The activity of caspase-3 was significantly up-regulated in 5 groups treated with quecetin as compared with control group （P〈0.05）. It was concluded that the growth inhibition of quercetin was highly related to cell cycle arrest at the G2/M phase and induction of caspase-dependent apoptosis in human nasopharyngeal carcinoma HEN 1 cells.
作者 张峰 崔永华 曹平平
机构地区 Department of Otorhinolaryngology-Head and Neck Surgery
出处 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2008年第3期369-372,共4页 华中科技大学学报（医学英德文版）
关键词 QUERCETIN HEN1 cells APOPTOSIS cell cycle CASPASE-3 quercetin HEN1 cells apoptosis cell cycle caspase-3
分类号 R739.63 [医药卫生—肿瘤]
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参考文献11

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Journal of Huazhong University of Science and Technology(Medical Sciences)
2008年 第3期

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