摘要
建立了铜绿假单胞菌(PA)随机扩增多态性DNA(RAPD)指纹图基因分型方法,并应用于流行病学相关或不相关的75个PA菌株的基因分型。分型率为100%。32株新生儿暴发感染菌株,可分成4个血清型和5个RAPD谱型,其中25株属于同一谱型。在8例患者的垂直追踪菌株中,除有1例其第1和第3个分离株的血清型和RAPD谱型均相同,但与第2个分离株的血清型和RAPD谱型不同外,其余7例前后菌株的谱型相同。13株散发菌株可分成3个血清型和10个RAPD谱型;12株流行病学无关菌株的RAPD谱型均不相同。本研究结果表明,RAPD指纹图基因分型法具有分型率高、分辨力强、比较快速简便等优点,并且不需要已知核酸序列,是在分子水平上对微生物感染的病原学、发病机理及流行病学研究的较理想的方法。
Randomly amplified polymorphic DNA fingerprinting was established and used to type 75 epidemiological related or unrelated strains of Pseudomonas aeruginosa .Typeability was 100%.Thirty two epidemic strains from the newborns were divided into 4 serotypes and 5 RAPD profiles;among which 25 strains had a same profile.The first and the third isolate from patient C were the same both in serotype and RAPD profile,but different from the second one both in serotype and RAPD profile.The follow up strains from each of 7 other patients had a same profile.Thirteen sporadic isolates were divided into 3 serotypes and 10 RAPD profiles.Twelve epidemiological unrelated strains were distinguishable each other in RAPD profiles.The results showed that RAPD fingerprint genotyping is suitable for studies on the etiology,pathogenesis and epidemiology of microbe infection at molecular level with high typeability,powerful discrimination,rapidness and simplicity.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第4期309-311,共3页
Chinese Journal of Microbiology and Immunology
基金
福建省卫生厅科学基金
关键词
指纹图
基因分型
铜绿假单胞菌
Fingerprint\ \ Genotyping\ \ Pseudomonas aeruginosa