HLA-DRB1*04等位基因亚型的DNA分型

DNA typing for alleles of HLA DRB1*04

采用顺序特异引物聚合酶链反应技术（ＰＣＲ－ＳＳＰ）检测我国汉族人群ＨＬＡ－ＤＲＢ１＊０４等位基因亚型频率，并与白种人群进行比较。利用５′端公用引物与３′端８个特异性引物进行ＰＣＲ反应，可准确分辨出１１个ＤＲＢ１＊０４等位基因，耗时４小时，无假阳性和假阴性结果。８５份随机样本的分型结果显示，我国汉族人群ＤＲＢ１＊０４基因亚型的频率与白种人之间存在非常显著性差异。

HLA DRB1*04 alleles of Chinese Hans population were determined by polymerase chain reaction with sequence specific primers(PCR SSP).The gene frequencies of DRB1*04 subtype in Chinese population were compared with those obtained in Caucasian population.Eleven alleles of HLA DR4 could be accurately distinguished using 5′ end of the public primer and 3′ end of 8 sequence specific primers in 8 PCR reactions per individual.The overall time of genotyping for DRB1*04 subtype was only 4 hours.No false positive or false negative typing results were obtained.The results in the random 85 DRB1*04 positive individuals showed that the gene frequencies of DRB1*04 alleles in Hans popualtion were significantly different from those in Caucasian population.