中国株庚型肝炎病毒(HGV)感染猕猴的实验研究

Macaca mulatta as an animal model for HGV infection

应用０．５ｍｌ含中国株庚型肝炎病毒（ＨＧＶ）的血清接种５只猕猴，５只猕猴于接种后１周血清ＨＧＶＲＮＡ均阳转；ＨＧＶＲＮＡ滴度在猴体内可高达１∶１０５，较接种血清中ＨＧＶＲＮＡ滴度（１∶１０２）高出许多，提示ＨＧＶ在猕猴体内复制。其中４只猕猴血清抗－ＨＧＶ阳转；４只出现ＡＬＴ异常。后用其中１只猕猴感染后４５天的血清给另２只猕猴接种，该２只猴也出现血清ＨＧＶＲＮＡ和抗－ＨＧＶ阳转及ＡＬＴ异常。本研究表明，中国猕猴有可能作为ＨＧＶ感染的动物模型。

Macaca mulatta were inoculated with 0.5ml HGV RNA positive serum collected from a patient with hepatitis G.HGV RNA of all 5 monkeys converted to positive within 1 week after inoculation and increased appearently.Anti HGV seroconvertion was found in 4/5 of the monkeys from 15 to 45 days postinfection,and 4/5 of the monkeys had abnormal levels of ALT after infection.A HGV RNA positive serum obtained from one of the infected monkeys was used to infect other two monkeys.HGV RNA and anti HGV seroconversion,and ALT elevation were also observed in these two monkeys.The results showed that the Macaca mulatta may be used as an experimental animal model for HGV infection.