冰冻组织芯片的研制及可行性探讨

Development and clinical application of frozen tissue microarray in lung cancer diagnosis

背景与目的应用自制模具进行冰冻组织芯片的研制及可行性探讨,为简单、经济、规范地进行蛋白及基因原位分析提供一种新方法。方法应用自制模具制备48点阵冰冻组织芯片。并经HE染色和EMA免疫组化染色,对其在形态学及基因表达分析方面的可行性进行评价。结果冰冻组织芯片OCT模块组织芯排列基本整齐,HE切片细胞的形态结构基本清楚;免疫组化染色组织形态基本完好,EMA呈棕黄色颗粒状;冰冻组织芯片EMA免疫组化染色结果与普通冰冻切片免疫组化结果比较,符合率为94.7%(18/19),二者比较差异无统计学意义(P>0.05)。结论我们冰冻组织芯片的研制方法是可行的,完全可以用于形态学观察和蛋白表达方面的研究。

Background and objective Traditional techniques in clinical diagnostic pathology have some limitations. Here we tried to develope frozen tissue microarray as a fast, simple and economical technique. Methods A total of 48 dots frozen tissue microarray were constructed in this study including primary 19 lung cancer samples and 5 normal lung tissue samples from primary lung cancer. Hematoxylin-Eosin and immunohistochemical stains were performed on the sections and we assess the feasibility on morphology and genetic expression. Results The cores on frozen tissue microarTay of OCT block were regularly arranged and had no shift and distort. After HE-stained, morphology of the cores was well preserved. They were answered for the request of research.Immunohistochemistry of array slides： we performed immunohistochemistry on the tumor tissue microarTay with antibodies for EMA. EMA staining is uniform across the sample and gives the expected cytochylema-associated staining. There is no background staining. We compared the staining of immunohistochemistry on section of frozen tissue microarray to the results on normal slides. The results showed the accord rates were 94.7% （18/19）, and there were no significant differences between them each other （P〉0.05）. Conclusion The frozen tissue microarray is feasible. HE and immunohistochemical stains showing this methodology will be useful for morphology and immunohistochemical based protein analyses.