儿童和青少年血浆自然发生的抗β淀粉样蛋白抗体的表达

Expression of naturally occurring plasma anti-β amyloid antibody in children and youngsters

目的探讨自然发生的抗β淀粉样蛋白（Aβ）抗体在儿童和青少年血浆中的表达。方法将28名1-30岁的健康人群按10岁为1个年龄段分为3组,采用转基因动物模型、组织淀粉样斑块免疫活性（TAPIR）分析和免疫印迹法,分析各组血浆抗Aβ抗体的存在和免疫特性。结果抗Aβ抗体总发生率为27.8%,各年龄组TAPIR阳性率差异无统计学意义;其免疫特性与中老年健康对照者相似。结论抗Aβ抗体的出现可能早于脑实质Aβ和老年斑的形成。

Objective To explore the expression of naturally occurring plasma anti-βamyloid （ Aβ ） antibody in children and youngsters . Methods According to each 10 years, 28 healthy cases aged from 1 - 30 years old were diviedd to 3 groups. In the 3 groups, the plasma anti-Aβ antibody was detacted by immunostained with paraffin sections from Tg2576 mice brain to make a specific tissue amyloid plaque immunoreactivity （TAPIR） and immunoprecipitation analysis. Results The total occurrence rate of anti-Aβ antibody was 27.8%. There was no significant difference of TAPIR positive rate in each age group. They had the similar immunospecialties to middleaged and elderly healthy controls. Conclusion Plasma anti-Aβ antibody can be occured before amyloid deposits and senile plaques in brain.