碱性成纤维细胞生长因子和银杏叶提取物对实验性脑梗死的作用

Effects of basic fibroblast growth factor and Gingkgo biloba extra on experimental cerebral infarction

目的探讨碱性成纤维细胞生长因子(bFGF)和银杏叶提取物(GbE)对实验性脑梗死的作用。方法健康家猫40只,随机分为生理盐水对照(A)组、bFGF治疗(B)组、GbE治疗(C)组和bFGF+GbE联合治疗(D)组;采用左侧眼眶入路制作猫大脑中动脉缺血再灌注模型;制模后即刻、24h、48h分别给予相应的药物干预;制模后1d、7d分别进行神经功能缺损评分;制模后7d对脑组织切片进行光镜和电镜下病理及超微结构观察。结果D组神经功能缺损评分(41.50±8.18)明显低于A组(82.20±8.08)、B组(63.60±6.22)和C组(61.00±6.58)(均P<0.01);光镜下D组神经细胞核轻度皱缩,细胞周围间隙大致正常,神经纤维网疏程度不严重;电镜下D组神经元轻度变性,细胞核无皱缩,核膜完整,线粒体轻度肿胀;脑组织病理损害程度较B组和C组明显减轻。结论bFGF和GbE联合治疗实验性脑梗死疗效优于二种药物的单一用药,两药合用具有脑保护的协同作用。

Objective To study the effects of basic fibroblast growth factor （bFGF） and Gingkgo biloba extra （GbE） on experimental cerebral infarction. Methods 40 healthy adult cats were equally devided into group A with sodiumchloride, group B with bFGF, group C with GbE and group D with bFGF ＋ GbE in random. All animals were operated to establish the feline models of cerebral focal ischemia-reperfusion by the approach of left transorbital. The certain madicine intervene were take at 0 h ,24 h ,48 h after the models established. The neurological defects score was evaluated by Philips＇ criteria score in 1 d and 7 d. The physiological changes were observed by HE stained and electron microscope. Results The neurological defects score was singificiant lower in D group （41.50 ±8.18 ） than that in group A（82.20 ±8.08） ,B（63.60 ±6.22） and C （61. 00 ±6.58） （all P 〈0. 01 ）. Compared with groups A, B,C, group D showed that the cellular nucleus was shrinkaged slightly,the gap of cells was nomal roughly and the degree of rarefaction in neurofibra net was not serious under the light microscope. Under the electron microscope,the neuron was degenerated, the cellular membrane was integrated and the plastosome was swelled lightly in the group D. The physiological demage were slighter in D group. Conclusions There is batter curative effect in bFGF combinated GbE than in single of bFGF or GbE to treat experimental cerebral infarction, and can enhance brain protection.