系统性红斑狼疮患者血清抗DNA酶B抗体检测的临床意义

Clinical Value of Examining Serum Anti-DNA Enzyme B in Patients with Systemic Lupus Erythematosus

目的探讨系统性红斑狼疮(SLE)患者血清中抗DNA酶B抗体滴度测定的临床意义。方法采用微量滴定法分别测定了SLE患者77例和正常人43例血清中的抗DNA酶B抗体滴度,并结合临床资料进行对比分析。结果SLE组抗DNA酶B抗体的阳性率为76.6%;对照组55.8%,2组差异有统计学意义(χ2=5.60,P=0.018)。血清抗DNA酶B抗体阳性的SLE患者发热、关节炎、肾损害的发生率均显著增高(χ2依次为4.084,5.502,8.606,P分别为0.043,0.019,0.003);血清CRP增高,补体C3降低以及抗ds-DNA抗体、抗核小体抗体阳性者亦显著增多(χ2依次为4.247,9.899,4.987,6.512,P分别为0.039,0.002,0.026,0.011)。SLE活动期和非活动期抗DNA酶B抗体的阳性率分别为67.8%和16.7%,活动期患者显著为高(χ2=14.62,P=0.000)。结论SLE患者中A组溶血性链球菌(GAS)感染发生率高于正常人,SLE合并肾损害时需要排除GAS感染。GAS感染与SLE病情活动有一定的关系。

Objective To investigate the clinical value of examining serum anti-DNA enzyme B antibody in patients with systemic lupus erythematosus（SLE）. Methods Anti-DNA enzyme B antibody in sera of 77 patients with SLE were examined by microtitration,The patients were divided into two groups according to anti-DNA enzyme B antibody being positive or negative, then contrasted and analysised. Results Positive rates of anti-DNA enzyme B antibody in systemic lupus erythematosus patients and control subjects were 76.6% , 55.8% respectively. There was a significant difference between the control and SLE. The occurrence rate of DNA enzyme B positive among SLE patients with fever, arthritis, the incidence of kidney damage were significantly higher（ X^2 were 4. 084,5. 502,8. 606, P were 0.043,0. 019,0.003 ） ; And serum CRP, complement C3 as well as the anti-ds-DNA antibody, the anti-nucleosome antibody positive rate were also obviously increased（ X^2 were 4.247,9. 899,4.987,6.512, P were 0. 039 ,0. 002 ,0. 026 ,0. 011 respectively）. SLE patients with active and non-active DNA B-positive rates were 67.8% and 16.7% , in patients with activities were significantly higher（ X^2 = 14.62 ,P = 0. 000 ）. Conclusions The occurrence rate of which group A streptococci（GAS） infected is higher in patients with SLE. SLE with renal damage needs to exclude GAS infection. The GAS infection and the SLE activity have certain relations.