人γδT细胞识别肿瘤抗原的分子机理研究

Studies on the Molecular Recognition of Tumor Autigens by γδ T Cells

作者在自建体外扩增人γδT细胞的基础上,分析了γδT细胞识别肿瘤细胞的分子机理.主要结果:(1)γδT细胞能够杀伤K562、Daudi、XG-7等肿瘤细胞,杀伤率分别高达62±7.2%,73±8.7%和84±6.1%;(2)γδT细胞对正常淋巴母细胞(自体和异体)的细胞毒活性均低于20%;(3)抗热休克蛋白70(HSP-70)单克隆抗体可阻断γδT细胞对XG-7细胞的杀伤作用,但对Daudi,K562肿瘤细胞却无明显的阻断作用.提示γδT细胞可通过识别肿瘤细胞表面的HSP或相关分子,并以MHC非限制性方式杀伤肿瘤细胞.

In this paper,we analysed the molecular mechanism by whichγδ T cells recognize and kill tumor cells on the basis of the established culture system for specific expansion of human 7 ST cell in our laboratory. The results were as follows: (1)γδT cells can recognize and mediate strong cytotoxic activity to different tumor cells including XG-7 ,K562,Daudi; (2)No cytotoxic activity to normal cells could be observed ; (3)Anti-HSP70 monoclonal antibody could block the cytotoxic activity of 7 ST cells against XG-7,but had no remarkable effect on K562 and Daudi cells. It suggests that γδ T cells may recognize HSP or the related molecules on tumor cell surface and mediated non MHC-restricted cytotoxicty.