康宁木霉菌株的诱变选育及固态发酵条件的优化

Mutation breeding of Trichoderma koningii and optimization of solid-state fermentation conditions

以康宁木霉3.4261为出发菌株,分别经紫外线诱变、紫外线与硫酸二乙酯复合诱变,采用刚果红透明圈法初筛和摇瓶复筛,获得1株高产纤维素酶的突变株E20-2,初步确定了固态发酵最佳产酶条件:麦麸︰秸秆=1:4,固:液=1:3～1:4,0.1%Tween-80,温度28℃、pH6.0、时间5d,纤维素酶(CMCase)、滤纸酶(FPA)酶活分别达到654U/g和69U/g,较出发菌株分别提高了230%和72%。

Trichoderma koningii 3.4261 was used as the original strain and treated with UV and EMS for mutation. One high cellulase-pmducing mutant strain named E20-2 was obtained by screening with Congo red transparent zone method and shake flask fermentation. Preliminary analysis showed the optimal fermentation conditions were： wheat bran： wheat straw of 1：4, solid： liquid of 1：3-1：4, Tween-80 concentrations 0.1%, temperature 28℃, pH 6.0, cultured for 5 days. The CMCase and FPAase of the mutant strain reached 654 U/g and 69 U/g, which were 230% and 72% higher than that of the parent strain, respectively. And E20-2 had a relatively higher cellulase producing ability than ever reported.