复合诱变选育新霉素高产菌株的研究

Screening of high neomycin-producing strains by compound mutation

为了获得优良的新霉素高产菌株,以弗氏链霉菌(Streptomyces fradiae)AS4.576为出发菌株,经4次紫外线诱变,紫外线-LiCl处理,随机挑选平板菌落214株,初筛得到13株抑菌圈较出发菌株>90%的菌株,经复筛获得1株效价提高178倍的菌株UV40s-9(2),再经微波复合诱变,以链霉素作为筛选因子得到菌株WB40s-6,其效价较UV40s-9(2)菌株提高了15.2%。

The original strain Streptomyces fradiae AS 4.576 was mutated with four series of ultra-violet and ultra-violet-LiCl treatment. 13 strains were obtained whose inhibition zone diameter increase 90% than the parent strain from 214 mutated strains. One high neomycin-producing strain UV40s-9 （2） was selected according to ambo-screen. Then UV40s-9（2） was mutated with microwave using streptomycin as the screening-factor. A mutant strain WB40s-6 was obtained, whose total titer was 15.2%, which was higher than that of UV40s-9 （2）.