骨肉瘤中微血管密度与预后的研究

Correlation of tumor microvesseldensity with prognosis in osteogenic sarcoma

目的探讨血管生成与骨肉瘤临床病理及预后的关系。方法通过内皮细胞特异性标记物ＦⅧ－ＲＡｇ和ＣＤ３１免疫组化染色，计数７０例骨肉瘤微血管密度。结果肿瘤微血管密度与骨肉瘤体积，Ｐｒｉｃｅ′ｓ分级和Ｄａｈｌｉｎ分型无关，而与肿瘤性骨样组织分型，ＷＨＯ新分型及ＰＣＮＡ标记指数有关。Ｋａｐｌａｎ－Ｍｅｉｅｒ分析微血管计数＜３１（×２００）的骨肉瘤预后较好，而＞３１（×２００）的骨肉瘤预后较差。结论结果表明骨肉瘤微血管密度与生存率相关，提示肿瘤血管生成分析可能是骨肉瘤一项有价值的预后指标。

Objective To investigate the relationship between tumor angiogenesis and clinical pathology, as wel as the prognosis in osteosarcoma. Methods Intratumoral microvessel density (MVD) in 70 cases of osteosarcoma was assessed immunohistochemically by using the specific endothelial cell markers FⅧRA and CD31.Results There was no correlation between the MVD and the tumor size, Price′s grade, as well as Dahlin′s classification; however, significant correlation was found between the MVD and the neoplastic osteoid classification,new WHO classification in 1993, as well as the proliferating cell nuclear antigen (PCNA) labelling index. Microvessel counts were associated with overall survival by KaplanMeier analysis. An average vessal count of less than 31(×200) suggested a better survival, but a higher vessel count of more than 31(×200) showed a trand to worse the overall survival. Conclusion The results suggest a significant relationship between MVD and prognosis; moreover, MVD may be a useful prognostic indicator in osteosarcomas.