TNF-α和M-CSF对骨巨细胞瘤溶骨的促进作用

Promotive effect of TNFα and MCSF on osteolysis induced by giant cell tumor of bone

目的探讨骨巨细胞瘤（ＧＣＴ）瘤组织细胞成分及其所分泌的细胞因子对骨质吸收的影响。方法从１０例ＧＣＴ中分离出多核巨细胞和单核基质细胞，并通过细胞培养获得纤维母细胞样基质细胞，在体外溶骨模型中检测这些细胞的骨吸收活性及细胞因子对这种活性的影响。结果所分离的ＧＣＴ细胞成分均有溶骨活性。外源性肿瘤坏死因子－α（ＴＮＦ－α）的加入能促进原代基质细胞的溶骨活性。ＧＣＴ组织中ＴＮＦ－α含量和巨噬细胞克隆刺激因子（Ｍ－ＣＳＦ）检出率比骨肉瘤和正常血清高。结论ＧＣＴ特征性的局部溶骨现象，是ＧＣＴ中三种主要的细胞成分共同作用于局部骨质的结果，而这些细胞所分泌的ＴＮＦ－α和Ｍ－ＣＳＦ促进了这一溶骨过程。

Objective To explore the effect of cell components in giant cell tumor of bone (GCT) and cytokines expressed by them on osteolysis. Methods Mononuclear stromal cells and multinucleated giant cells (MGCs) were isolated from 10 cases of GCT, and fibroblastlike stromal cells were obtained by longterm culture of mononuclear stromal cells. Osteolytic capability of above isolated cells were tested in an in vitro cellbone resorption model. Results All cell components isolated from GCT had capability to resorb bone matrix directly. Exogenous tumore necrosis factorα(TNFα) could significantly increase the bone resorption indcued by both kinds of stromal cell. There were higher level of TNFα and greater expression rate of macrophage colony stimulating factor(MCSF) in GCT than in the osteosarcoma tissues or the normal serum. Conclusions The characteristic bone resorption behavior of GCT might be conducted by all it′s three major cell components, and this bone resorption process could be promoted by both TNFα and MCSF they expressed.