碱性铁蛋白对肾小球基膜及系膜区通透性的影响

The effect of cationic ferritin to the permeability of renal glomerular basement membrane and the mesangial area

目的为探讨碱性铁蛋白（ＣＦ）注射后，对肾基膜（ＧＢＭ）及系膜区通透性的影响。方法用带有阳性电荷的ＣＦ，按５０ｍｇ／ｋｇ的量一次性由静脉注入大白鼠体内。取肾组织经聚乙烯亚胺（ｐｏｌｙｅｔｈｙｌｅｎｉｍｉｎｅ，ＰＥＩ）特殊染色，在电子显微镜下观察。结果基膜的ＰＥＴ颗粒数量显著改变（Ｐ＜０．０５），而系膜基质的ＰＥＩ却无变化（Ｐ＞０．０５）。结论基膜的负电荷起着电荷屏障作用，与蛋白尿的产生有关；而基质区域则是淋巴通路，与蛋白尿的形成无关。

Ojbective To study the effect of cationic ferritin (CF) to the permeability of the glomerular basement membrane (GBM) and the mesangial area. Methods Single dose of CF 50mg/kg/rat given intravenously. Samples taken from the kidney were studied under electronic microscope by polyethylenimine (PEI) staining. Results The number of PEI granules in GBM changed obviously while those in the mesangial matrix (MM) remained unchanged. Conclusion The negative charge in GBM serves as a charge barrier relating with proteinuria and the MM serves as the lymphatic pathway with no connection with proteinuria.