氨基胍对兔骨关节炎软骨细胞凋亡的影响

The effect of aminoguanidine intraperitoneal injection on apoptosis in chondrocyte with osteoarthritis

目的研究氨基胍对兔骨关节炎软骨细胞凋亡的影响。方法将32只兔子随机分组,正常组未采取任何处理,氨基胍组及模型组行右后肢伸直位管型石膏固定造模,氨基胍组腹腔内注射100 mg/(kg.d)氨基胍硫酸盐溶液,模型组每天腹腔内注射相同体积的生理盐水。分别于4周和8周处死动物,留取标本观察软骨大体形态,切片HE染色观察镜下形态,免疫组化检测iNOS表达,TUNEL法原位检测凋亡。结果与模型组相比,氨基胍组在大体形态、HE切片镜下形态接近于正常组,组化染色显示iNOS表达阳性细胞数显著减少(P<0.05),TUNEL法原位检测显示凋亡阳性细胞数显著减少(P<0.05)。结论氨基胍能有效减少iNOS表达和软骨细胞的凋亡,延缓OA的病情发展。

Objective To observe the effect of aminoguanidine by intraperitoneal injection on apoptosis of chondrocyte with osteoarthritis. Methods Totally 32 rabbits were divided into 3 groups at random. Both the experiment and the model groups were fixed by plaster cast in the right hind straighten limb, while the control group received no treatment. The experiment groups were injected with aminoguanidine in abdominal cavity, 100 mg/（kg · d）, and the control groups were injected the same voluminal normal saline in abdominal cavity. At the 4th week, we killed 4 rabbits from the normal group, and 6 from the control group and the experiment group, respectively. At the 8th week, we put the rest to death. We drew the materials from each sample for pathological observation on the whole and under the light microscope by HE and immunohistochemical staining, and counted apoptosis cells by TUNEL for statistical analysis. Results Compared with the control group, the experiment group was more similar to the normal group in general observation and optical microscope observation by HE staining. Both iNOS positive expression cell by immunohistochemical staining and apoptosis cell calculation by TUNEL of the experiment group were fewer than those of the control group（P〈0.05）. Conclusion Aminoguanidine injected in abdominal cavity can depress the production of iNOS, decrease apoptosis of the cartilage cell, and delay or prevent progression of OA.