维生素E对糖尿病大鼠高级糖基化终末产物及其受体和肾功的影响

Effect of Vitamin E on advanced glycosylation end-products and their receptors in renal tissue of diabetic rats

目的观察维生素E对糖尿病大鼠高级糖基化终末产物(AGEs)及其受体(RAGE)和肾功能的影响。方法利用链脲佐菌素腹腔注射法诱导建立糖尿病大鼠模型。将实验用SD大鼠随机分为正常对照组(NC组)、糖尿病组(DM组)和维生素E治疗组(VE组),治疗8周。观察大鼠的一般状况,监测血糖(BGL)、糖化血红蛋白(HbA1C)、尿素氮(BUN)、血肌酐(Scr)、内生肌酐清除率(Ccr)、尿白蛋白排泄率(UAER)、肾重/体重比值;应用荧光显微镜观察肾脏组织AGEs,免疫组化方法检测RAGE含量。结果①与NC组比较,DM组大鼠体重、血糖、HbA1C、BUN、UAER、肾重/体重比值均明显增高(P<0.01),Ccr明显下降(P<0.01),肾脏AGEs及RAGE含量均明显增高(P<0.01);②与DM组相比,VE组UAER、肾重/体重比值明显降低(P<0.05),Ccr明显升高(P<0.01),肾脏AGEs及RAGE含量均明显降低(P<0.05)。结论维生素E无降糖作用,但可能通过减轻糖尿病大鼠肾组织的非酶糖基化过程,从而减轻AGEs与受体结合后导致的对肾脏组织的损伤,对糖尿病大鼠肾脏具有保护作用。

Objective To observe the effect of Vitamin E on advanced glycosylation end-products （AGEs） and their receptors in renal tissue of diabetic rats. Methods Rat diabetic nephropathy was induced by intraperitoneal injection of STZ. Rats were allocated to normal control group （NC group）, diabetes mellitus group （DM group）, and Vitamin E group （VE group）. All rats were treated with corresponding drugs for 8 weeks. During and after the treatment, the general state, blood glucose level （BGL）, blood urea nitrogen （BUN）, serum creatinine （Scr）, urinary albumin excretion rate （UAER）, glycosylated hemoglobin （HbA1C）, clearance rate of creatinine （Ccr） and kidney weight/body weight ratio were determined in different groups. The fluorescence microscope was used to observe advanced glycosylation end-products fluorescence intensity in iced slices. The expression of RAGE in rats＇ renal tissue slices was measured by immunohistochemistry. Results （1） Compared with those in NC group, GLU, HbA1C, BUN, UAER, kidney weight/body weight ratio, contents of AGEs, and RAGE increased significantly （P〈0.01）, while Ccr decreased significantly （ P 〈 0.01） in DM group. （2） Compared with those in DM group, UAER, kidney weight/body weight ratio, contents of AGEs, and RAGE decreased significantly （P〈0.05）, while Ccr significantly （P〈0.05） increased in VE group. Conclusion Vitamin E has no effect in decreasing blood glucose level, but has a certain role in preventing renal function by alleviating the accumulation of advanced glycosylation end-products.