早期自然流产患者绒毛组织中核转录因子-kappa B的表达

Expression of nuclear factor κB in the tissuses of chorion of early spontaneous abortion

目的探讨核转录因子-κB(NF-κB)在绒毛组织中的表达及活化与早期自然流产的关系。方法采用免疫组化方法检测早期自然流产患者和同期妊娠的健康妇女绒毛组织中NF-κB p65的表达;激光共聚焦显微镜(CLSM)检测NF-κB p65的核区与胞浆区比值(FN/FC)以观察其活化情况。结果早期自然流产患者绒毛合体滋养层细胞NF-κB表达强度低于正常早期妊娠妇女,其差异具有统计学意义(P<0.001);早期自然流产患者绒毛间质细胞NF-κB表达的FN/FC(0.7602)明显高于正常早期妊娠妇女(0.6977)(t=3.511,P<0.001)。结论NF-κB在早期自然流产患者绒毛组织合体滋养细胞中的低表达以及其在间质细胞中的过度活化可能与早期自然流产的发生有关。

Objective To examine the cellular distribution and expression, activation of nuclear factor κB （NF-κB） in the tissuses of chorion of early spontaneous abortion, and explore the relationship between early spontaneous abortion and NF- κB. Methods The NF- κB expression on the chorionic villi was analyzed by S-P immunohistochemistry. The nucleoplasmic ratio of NF-κB was detected by laser scanning confocal microscope. Results The expression intensity of NF- κB in the syncytiotrophoblasts was significantly weaker in women with early spontaneous abortion than in the normal early pregnancy （P〈0. 001）. Significant difference existed in the nucleoplasmic ratio of NF-κB expression in stromal cells of chorionic villi between early spontaneous abortion （0.7602） and normal early pregnancy （0.6977）（t=3.511, P〈0.001）. Conclusion Low expression of NF- κB in stromal cells of chorionic villi and the excessive activation of NF-κB in villus stromal cells are significantly correlated with the occurrence of early spontaneous abortion.