碘酸钾对大鼠肝脏抗氧化能力影响

Effect of potassium iodate on anti-oxidative capability of liver in rats

目的观察Wistar大鼠补充碘酸钾(KIO3)对肝脏抗氧化能力的影响。方法将Wistar大鼠随机分为6组:低碘组(LI),适碘组(NI),5倍高碘组(5HI),10倍高碘组(10HI),50倍高碘组(50HI),100倍高碘组(100HI)。喂养3、6和12个月后,检测肝组织匀浆中的谷胱甘肽过氧化酶(GPx)活性、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量。结果LI组GPx活性低于NI组(P<0.01),但SOD活性较NI组增高(P<0.05),MDA含量则较NI组增高(P<0.01)。10HI组的SOD活性在6和12个月时增高(P<0.05),50HI和100HI组的SOD活性在3个月时较NI组显著增高(P<0.01),但在6和12个月时均较NI组显著降低(P<0.01)。4个HI组的GPx活性和MDA含量与NI组差异均无统计学意义(P>0.05)。结论低碘导致大鼠肝脏抗氧化损伤;长期摄入高剂量碘酸钾未见对肝脏产生明显过氧化损伤,仅于10倍、50倍和100倍高碘组抗氧化酶活性降低。

Objective To study the effect of KIO3 on liver anti - oxidative capability. Methods The rats were divided into 6 groups and given by different dosage of KIO3 with low iodide （LI）, normal iodide （NI）, 5 folds high iodide （SHI）, 10 folds high iodide （10HI）, 50 folds high iodide （50HI） and 100 folds high iodide （100HI）. 3, 6 and 12 months later, the rats were sacrificed and liver GPx activity, SOD activity and MDA content were measured. Results The GPx activity in LI group was markedly lower than NI group. Furthermore, the MDA content and the SOD activity of LI group was higher than that of NI group. The SOD activity of 10HI group was higher than that of NI group after administration for 6 and 12 months, but no difference was found after administration for 3 month. The SOD activity of 50HI and 100HI groups were higher than that of NI group after administration for 3 months, but lower than that of NI group after administration for 6 and 12 months. There was no difference in GPx activity and MDA content between NI group and 4 high iodide groups. Conclusion Low iodide intake would damage anti - oxidative capability of liver in normal rats. There was no obvious oxidative damage to liver in the rats after administration by high iodate for a long period.