植物单宁对K562细胞诱导红系分化抑制作用

Inhibitory effect of plant tannins on butyric acid and hemin-induced erythroid differentiation of K562 cells

目的研究可水解单宁诃子酸和特里马素I对氯化高铁血红素和丁酸钠诱导K562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素I对K562细胞生长的影响,联苯胺染色法检测血红蛋白合成情况,应用免疫荧光和流式细胞术检测血型糖蛋白A（GPA）在细胞表面的表达。结果诃子酸和特里马素I在0.04～0.09 mmol/L浓度下均可显著抑制K562细胞的生长增殖。2种单宁化合物（0.002～0.01 mmol/L）可显著抑制丁酸钠诱导的K562细胞血红蛋白合成,2种单宁化合物（0.01～0.05 mmol/L）还可显著抑制氯化高铁血红素诱导的血红蛋白合成,特里马素I（0.01 mmol/L）还抑制丁酸钠诱导的GPA在细胞表面表达。结论诃子酸和特里马素I对红系分化有抑制作用。

Objective Effects of plant tannins, chebulinic acid and tellimagrandin I on chemically induced hemoglobin synthesis in K562 cells were investigated. Methods The hemoglobin synthesis situation was assayed with benzidine staining. Erythroid antigens glycophorin A（ GPA） expression on the surface of K562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate - conjugated anti - GPA antibodies. Then flow cytometric analysis was performed to detect GPA expression levels on surface of the cells. Results Both chebulinic acid and tellimagrandin I could inhibit the hemoglobin synthesis Of butyric acid and hemin - treated K562 cells in a concentration - dependant manner. However, the BA - induced K562 cells were more sensitive to two tannins than heroin - induced cells. Conclusion Chebulinic acid and tellimagrandin I have inhibitory effect on the erythroid differentiation.