体外生物人工肝支持系统的构建及其意义

ESTABLISHMENT AND SIGNIFICANCE OF AN EXTRACORPOREAL BIOARTIFICIAL LIVER SUPPORT SYSTEM

为建立理想的体外生物人工肝支持系统（ＥＢＬＳＳ），给肝衰竭患者提供新的治疗手段和措施，应用两步灌流技术分离人肝细胞，球形聚集法加以培养，同时使用中空纤维型生物反应器和血液透析仪改装的辅助循环系统，共同构成体外生物人工肝支持系统，并以正常犬为对象进行体外实验。结果显示，分离所得肝细胞成活率为９４％，总产量达２×１０１０个以上，肝细胞在限制贴壁条件下形成球形聚集体悬浮生长，ＥＢＬＳＳ在实验犬体外运行５小时后，肝细胞仍保持较高的活性。实验过程犬生命征平稳，血清总蛋白和白蛋白增加。上述结果表明，所建ＥＢＬＳＳ已基本解决了生物人工肝的关键技术，系统安全、有效。为进一步开展肝衰竭的生物人工肝支持研究奠定了基础。

This experiment was carried out to establish an optimal extracorporeal bioartificial liver support system (EBLSS) and furnish a new therapy for hepatic failure. For this purpose, human hepatocytes were isolated by two stage perfusion method and cultured by spheroidal aggreagates method. Besides, hollow fiber tube was used as a bioreactor and the hemodialysis instrument was refited to serve as supplementary circulation unit, which was composed of EBLSS and was tried in normal dogs. A total of 2×10 10 human hepatocytes with a viablity of 94% was obtained. These hepatocytes became multicellular spheroidal aggregates under non adherent conditions. After 5 hours circulating on EBLSS, the hepatocytes remained active. At the same time, the monitoring system revealed a normal vital sign in dogs and the laboratory tests found their serum total protein and albumin were increased. These results show that the key tech nology of bioartificial liver has been dereloped basically and this EBLSS is effective and safe, providing a foundation for the support and treatment of hepatic failure with bioartificial liver.