摘要
以NaCl、Na2SO4、MgSO4和(NH4)2SO4为盐析剂,对影响羔羊皱胃酶的盐析饱和度、盐析pH、盐析温度和盐析时间进行了研究.结果表明,不同盐析剂对羔羊皱胃酶盐析效果各不相同,(NH4)2SO4是盐析羔羊皱胃酶较适宜的盐析剂.(NH4)2SO4饱和度95%、盐析pH值为4.60、盐析温度为4℃下盐析14-20h,盐析活性回收率可达到92.86%.
Using NaCl, Na2SO4 、 MgSO4 and (NH4) 2 SO4 as salting-out reagents, some factors, such as saturation pH, temperature and time, influencing salting-out effects on kid rennet were studied systematically. The results showed that different salting-out reagents have different effect on salting-out and (NH4)2SO4 is a optimal salting-out reagent,by which enzyme activity recovery can reached 92.86% under (NH4)2SO4 saturation 95%, pH 4.60, temperature 4℃ and time 14-20 hours.
出处
《陕西师范大学学报(自然科学版)》
CAS
CSCD
北大核心
2008年第4期106-108,共3页
Journal of Shaanxi Normal University:Natural Science Edition
基金
国家“十一五”科技支撑计划奶业重大专项研究基金资助项目(2006BAD04A11)
关键词
盐析剂
羔羊皱胃酶
盐析条件
salting-out reagent
kid rennet
salting-out condition