不同盐析剂对羔羊皱胃酶盐析效果的影响

Influences of different salting-out reagents on effect of kid rennet

以NaCl、Na2SO4、MgSO4和（NH4）2SO4为盐析剂，对影响羔羊皱胃酶的盐析饱和度、盐析pH、盐析温度和盐析时间进行了研究．结果表明，不同盐析剂对羔羊皱胃酶盐析效果各不相同，（NH4）2SO4是盐析羔羊皱胃酶较适宜的盐析剂．（NH4）2SO4饱和度95％、盐析pH值为4．60、盐析温度为4℃下盐析14-20h，盐析活性回收率可达到92．86％．

Using NaCl, Na2SO4 、 MgSO4 and （NH4） 2 SO4 as salting-out reagents, some factors, such as saturation pH, temperature and time, influencing salting-out effects on kid rennet were studied systematically. The results showed that different salting-out reagents have different effect on salting-out and （NH4）2SO4 is a optimal salting-out reagent,by which enzyme activity recovery can reached 92.86% under （NH4）2SO4 saturation 95%, pH 4.60, temperature 4℃ and time 14-20 hours.