EB病毒BHRF1基因表达对鼻咽癌细胞增殖能力的影响

The effect of the expression of BHRF1 gene of EB virus on the proliferation of the cells of nasopharyngeal carcinoma

为了解ＥＢ病毒编码基因ＢＨＲＦ１的表达对鼻咽癌细胞增殖能力的影响，通过构建携有ＢＨＲＦ１的高表达载体，并转染低分化鼻咽癌细胞株ＣＮＥ２细胞，观察转染后的癌细胞在缺乏营养条件下增殖能力的改变。结果发现：ＢＨＲＦ１的表达能抑制增殖核抗原的表达，并促进其在缺乏营养条件下生存能力。提示ＥＢ病毒编码的ＢＨＲＦ１基因可能与鼻咽癌的发生和发展过程有关。

In order to learn the effect of the expression of BHRF1 of Epstein Barr virus on the growth of the cell of nasopharyngeal carcinoma, high BHRF1 expressive vector was set up and trans fected in low grade cell line of nasopharyngeal carcinoma, CNE2. Then the cells were cultivated under the condition of insufficient nutrition. The results show that the BHRF1 expression can prohibit the expression of PCNA, and enhance the survival of tumor cells under conditions of nutrition deprivation . So, it suggests that BHRF1 of EB virus could be involved in the development of nasopharyngeal carcinoma.