蒲公英的组织培养研究

Tissue Culture of Dandelio

以盆栽蒲公英的叶柄和叶片为试材,对蒲公英进行组织培养。结果表明:300mg/L的抗坏血酸能够较好地防止褐化,直接诱导再生植株的最佳组合是MS+0.5mg/L6-BA+0.1mg/LNAA;诱导愈伤组织和继代的最佳组合是6-BA0.5mg/L+2,4-D0.5～1.0mg/L;1/2MS+15g/L蔗糖+NAA0.1mg/L是诱导生根的理想培养基。

With pot-cultured dandelion as test material, the leaf stalk and blade were used as explants for tissue culture. Addition of 300 rag/1 of vitamin C into the culture medium was most effective to prevent callus from browning, and the medium MS ＋ 0.5 mg/1 6-BA ＋ 0.1 mg/1 NAA is an optimum combination for direct regeneration. For indirect regeneration fragile calli were obtained from explants on the medium supplemented with 6-BA 0.5 mg/1 and 2,4-D 0.5-1.0 mg/1. The medium 1/2 MS ＋ NAA 0.1 mg/1 was suitable for rooting.